Skip to main content
. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: Dev Biol. 2014 Jan 21;387(2):167–178. doi: 10.1016/j.ydbio.2014.01.010

Fig. 3.

Fig. 3

Dkk2/Frzb antagonizes Wnt signaling. (A) Wnt reporter assay in HEK 293T cells. Super-TOPFLASH, a Wnt responsive reporter was co-transfected into HEK293T cells together with Wnt1 and other plasmids as indicated. hDkk1 (human Dkk1) was used as a positive control. Fold induction of Wnt reporter activity is shown. The numbers for each gene indicates the amount of DNA transfected; total amount of DNA transfected in each well was 150 ng, adjusted with pCS2+ plasmid. (B) Anteriorization of Xenopus embryos by injected mRNAs as indicated. mRNAs of chicken Dkk2 (1 ng per embryo), Dkk3 (6 ng per embryo) or Frzb (2 ng per embryo) were injected at 4-cell stage. The numbers of embryos with indicated phenotypes are also shown. (3/33) stands for 3 out of 33 injected embryos showed indicated phenotype. Control animals were injected with 250 pg mRNA of preprolectin gene. (C) Summary of the Xenopus injection experiment.