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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: Dev Biol. 2014 Jan 21;387(2):167–178. doi: 10.1016/j.ydbio.2014.01.010

Fig. 4.

Fig. 4

Overexpression of Dkk2/Frzb, but not Dkk3, disrupts feather regeneration. (A–D) Representative samples of lentivirus (LV) mediated gene overexpression in the feather follicle. A control virus carrying GFP only was also shown. Four to five wing flight feather follicles in their resting phase were plucked to induce regeneration and infected with the virus, and photographed 3 weeks afterwards. Delayed feather regeneration was indicated by arrow heads. (E–H) H&E analysis of virus-infected feather follicles 4 days post-infection. (I–L) pY489 β-catenin antibody staining (red spots) showing the reduced Wnt signaling after Dkk2/Frzb overexpression. The epithelia in the collar regions are shown, and the DP regions are shown in inserts. (M and N) Anti-GFP immunostaining (red) showing lentiviral-mediated GFP expression in the feather follicles. (O and P) Quantification of BrdU and TUNEL staining results in the feather follicles. The dp, Pp and collar (cl) are labeled; * indicates the disrupted structures. Bar = 1 cm (A–D, shown in D), 1 mm (E–H, shown in H; M and N, shown in N), and 200 μm (I–L, shown in L).