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. 2015 Mar 30;208(7):897–911. doi: 10.1083/jcb.201411124

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© 2015 Dudin et al.

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Figure 4. — Type V myosins Myo52 and Myo51 are essential for cell fusion. (A) Fusion efficiency of indicated heterothallic crosses. myoVΔ (=myo51Δ myo52Δ double mutant) is unable to fuse with either fus1Δ or itself. The total number of mating pairs analyzed (three experiments combined) is indicated on the right. (B) Homothallic h90 wild-type, myo51Δ, myo52Δ, and myoVΔ strains expressing Fus1-sfGFP. Images are time-averaged projections over time 15 min at 1 image/min. Type V myosins are important for Fus1 focalization. (C) Quantifications of Fus1-sfGFP zone size in strains as in B; n = 12. (D) Crosses of h⁺ wild-type (WT) and myoVΔ strains expressing GFP-CHD to h⁻ myo52-tdTomato. Images are time-averaged projections over 15 min at 1 image/min. Type V myosins are important for actin focalization at the fusion site. (E) Asci derived from homothallic h90 myo52Δ and myo51Δ matings. We observed residual cell wall (blue arrowhead) and narrow necks (green arrowhead) in myo52Δ and myo51Δ, respectively. (F) Percentage of asci with residual cell wall and mean neck width in strains as in E. n > 200. (G) Crosses of h⁻ myo51^Δtail-3YFP (top) and h⁻ myo52^Δtail-tdTomato (bottom) to h⁺ wild-type cells. Both truncated motors localize correctly to the fusion focus. (H) Fusion efficiency of indicated heterothallic crosses. Note that a lower fusion efficiency is observed for h⁺ fus1Δ × h⁻ wild type than for h⁻ fus1Δ × h+ wild type in A. n > 100. Bars, 1 µm. Error bars are standard deviations.