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. 2015 Mar 30;208(7):949–959. doi: 10.1083/jcb.201404092

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Figure 7. — miR-501-3p expression is regulated locally in dendrites. Cultured hippocampal neurons (17 DIV) were treated with NMDA (30 µM for 5 min) alone or along with designated inhibitors and fixed at 90 min after treatment for in situ hybridization. (A) Subcellular localization of miR-501-3p. (B) Representative images of dendrites from transfected neurons. (C) Quantification of B; n = 15–23 neurons for each condition. (D) Effects of AMPAR and NMDAR blockers on NMDA-induced changes in mature miR-501-3p; n = 4–9 experiments for each condition. Bars: (A) 20 µm; (B) 5 µm. Data are shown as mean ± SEM; Mann-Whitney U test was used for statistical analysis. *, P < 0.05; ***, P < 0.005.