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. 2015 Mar 30;208(7):913–929. doi: 10.1083/jcb.201411047

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© 2015 Somasekharan et al.

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Figure 7. — G3BP1 kd inhibits local invasion and lung metastasis. (A) H&E-stained representative sections of implantation site tumors from NOD/SCID mice bearing renal subcapsular tumor xenografts of MNNG cell lines with (shG3BP1) or without (shControl) G3BP1 kd. Arrowheads show highly invasive growth pattern of shControl tumors (left) and noninvasive borders of shG3BP1 tumors (right). (B) H&E-stained sections of MNNG shControl and shG3BP1 tumors, with asterisks showing microscopic areas of necrosis. The latter is quantitated (bar graph, right) by measuring average percent geographical necrosis in 20 high-power fields from each tumor type (n = 5 tumors per group). Mean values ± SD (error bars) are shown. *, P < 0.05. (C) H&E-stained lung sections from mice bearing renal subcapsular tumor xenografts of MNNG cells with (shG3BP1) or without (shControl) G3BP1 kd. The arrowhead (left) shows a metastatic pulmonary lesion. The bar graph shows the total number of mice with at least one lung metastasis (Mets positive, red) in the indicated groups (n = 8 mice/group). Mean values ± SD (error bars) are shown. *, P < 0.05. (D) Primary site tumors with (shG3BP1) or without (shControl) G3BP1 kd were stained with antibodies to the Ki67 proliferation marker. The percentage of Ki67-positive cells in each group is quantitated in the bar graph (right) by counting cells in 20 high-power fields from each tumor type (n = 5 tumors per group). ns, nonsignificant. Error bars indicate SD. Bars, 100 µm.