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. 2015 Mar 30;208(7):881–896. doi: 10.1083/jcb.201412085

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© 2015 Moudgil et al.

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Figure 3. — Inhibition of farnesylation abrogates KT localization of hSpindly but not the RZZ complex. (A and B) HeLa cells were treated with 10 µM of L744832 FTI or DMSO for 24 h, fixed, and immunostained for hSpindly, Rod, and Zw10. Complete loss of hSpindly KT localization and normal Rod and Zw10 levels on the KTs were observed in FTI-treated cells. ACA immunostaining labels centromeres and DAPI stains DNA. (C) HeLa cells were treated with FTI or DMSO for 24 h and incubated with 0.5 µM vinblastine for 30 min before harvesting, showing no hSpindly KT localization without affecting Rod KT localization. (D) Normalized hSpindly protein expression from HeLa cells show 25% less expression with FTI treatment as compared with DMSO. n = 3. Error bars are SD from the means. P-value indicated a significant difference. Bars, 10 µm.