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. 2015 Mar 30;208(7):881–896. doi: 10.1083/jcb.201412085

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© 2015 Moudgil et al.

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Figure 5. — hSpindly farnesylation motif can be substituted with CENP-E or CENP-F farnesylation motif but not a geranylgeranylation motif. (A) A schematic diagram of hSpindly depicting substitution of its farnesylation motif with CENP-E and CENP-F, referred to as Spindly-E and Spindly-F, respectively. The last amino acid of Spindly is changed such that it is a geranylgeranylation motif instead of farnesylation motif referred to as Spindly-GG1 and Spindly-GG2. The KT localization of each construct is shown (+, KT localization; −, non-KT localization). Amino acid numbers are indicated. (B) HeLa cells were transiently transfected with EGFP-hSpindly fusion constructs (shown in A) and KT localization ability of each construct was analyzed using fluorescence microscopy. Representative images show that the farnesylation motif of Spindly can be replaced with the farnesylation motif of known farnesylated proteins CENP-E and CENP-F, but a geranylgeranylation motif cannot target hSpindly to KTs. Bar, 10 µm.