Table 2.
Mononegavirales-related RdRp sequences discovered in this study
| Virus name | Length of RdRp | Classification | Pool | Abundance | Putative arthropod host | Closest relative (aa identity) |
|---|---|---|---|---|---|---|
| Bole Tick Virus 3 | 2155 | Chuvirus | Ticks | 202.35 | Hyalomma asiaticum | Midway virus (17.1%) |
| Changping Tick Virus 2 | 2156 | Chuvirus | Ticks | 185.73 | Dermacentor sp | Midway virus (17.6%) |
| Changping Tick Virus 3 | 2209 | Chuvirus | Ticks | 41.80 | Dermacentor sp | Midway virus (16.5%) |
| Lishi Spider Virus 1 | 2180 | Chuvirus | Spiders | 5.82 | Parasteatoda tepidariorum | Midway virus (16.9%) |
| Shayang Fly Virus 1 | 2459 | Chuvirus | True flies | 8.99 | Atherigona orientalis | Maize mosaic virus (16.8%) |
| Shuangao Fly Virus 1 | 2097 | Chuvirus | Insect mix 1 | 23.63 | Unidentified Diptera | Lettuce big-vein associated virus (16.3%) |
| Shuangao Insect Virus 5 | 2291 | Chuvirus | Insect mix 1 | 209.31 | Unidentified Diptera, Abraxas tenuisuffusa, unidentified Chrysopidae | Potato yellow dwarf virus (16.3%) |
| Shuangao Lacewing Virus | 2145 | Chuvirus | Insect mix 1 | 44.48 | Unidentified Chrysopidae | Potato yellow dwarf virus (16.8%) |
| Tacheng Tick Virus 4 | 2101 | Chuvirus | Ticks | 137.22 | Argas miniatus | Midway virus (17.5%) |
| Tacheng Tick Virus 5 | 2201 | Chuvirus | Ticks | 276.32 | Dermacentor marginatus | Midway virus (16.8%) |
| Wenzhou Crab Virus 2 | 2208 | Chuvirus | Crabs and barnacles | 4054.25 | Charybdis japonica, Charybdis lucifera, Charybdis hellerii | Midway virus (15.8%) |
| Wenzhou Crab Virus 3 | 2077 | Chuvirus | Crabs and barnacles | 169.21 | Charybdis japonica | Midway virus (16.3%) |
| Wuchang Cockroach Virus 3 | 2203 | Chuvirus | Cockroaches | 440.14 | Blattella germanica | Midway virus (16.3%) |
| Wuhan Louse Fly Virus 6 | 2182 | Chuvirus | Insect mix 2 | 4.12 | Unidentified Hippoboscidae | Midway virus (16.4%) |
| Wuhan Louse Fly Virus 7 | 2174 | Chuvirus | Insect mix 2 | 99.83 | Unidentified Hippoboscidae | Midway virus (17.2%) |
| Wuhan Mosquito Virus 8 | 2159 | Chuvirus | Mosquito Hubei | 300.33 | Culex tritaeniorhynchus, C. quinquefasciatus, Anopheles sinensis, Armigeres subalbatus | Midway virus (16.7%) |
| Wuhan Tick Virus 2 | 2189 | Chuvirus | Ticks | 154.46 | Rhipicephalus microplus | Midway virus (16.7%) |
| Culex tritaeniorhynchus rhabdovirus | 2142 | Culex tritaeniorhynchus rhabdovirus | Mosquito Hubei | 3517.32 | Culex tritaeniorhynchus, C. quinquefasciatus, Anopheles sinensis, Armigeres subalbatus, Aedes sp | Isfahan virus (38.5%) |
| Wuhan Insect Virus 4 | 2105 | Cytorhabdovirus | Insect mix 4 | 94.92 | Hyalopterus pruni OR Aphelinus sp | Lettuce necrotic yellows virus (40.6%) |
| Wuhan Insect Virus 5 | 2098 | Cytorhabdovirus | Insect mix 4 | 622.97 | Hyalopterus pruni OR Aphelinus sp | Persimmon virus A (47.9%) |
| Wuhan Insect Virus 6 | 2079 | Cytorhabdovirus | Insect mix 4 | 991.99 | Hyalopterus pruni OR Aphelinus sp | Persimmon virus A (45.2) |
| Wuhan Louse Fly Virus 5 | 2123 | Kolente virus like | Insect mix 2 | 98.92 | Unidentified Hippoboscidae | Kolente virus (54.5%) |
| Yongjia Tick Virus 2 | 2113 | Nishimuro virus like | Ticks | 13.14 | Haemaphysalis hystricis | Nishimuro virus (54.2%) |
| Shayang Fly Virus 2 | 2170 | Sigmavirus like | True flies | 36.83 | Musca domestica, Chrysomya megacephala | Isfahan virus (44.1%) |
| Wuhan Fly Virus 2 | 2134 | Sigmavirus like | True flies | 18.37 | Musca domestica, Sarcophaga sp | Vesicular stomatitis Indiana virus (43.4%) |
| Wuhan House Fly Virus 1 | 2098 | Sigmavirus like | True flies | 31.04 | Musca domestica | Isfahan virus (42.8%) |
| Wuhan Louse Fly Virus 10 | 2146 | Sigmavirus like | Insect mix 2 | 235.94 | Unidentified Hippoboscidae | Drosophila melanogaster sigmavirus (51.2%) |
| Wuhan Louse Fly Virus 8 | 2145 | Sigmavirus like | Insect mix 2 | 292.11 | Unidentified Hippoboscidae | Drosophila melanogaster sigmavirus (50.6%) |
| Wuhan Louse Fly Virus 9 | 2145 | Sigmavirus like | Insect mix 2 | 69.37 | Unidentified Hippoboscidae | Drosophila melanogaster sigmavirus (51.4%) |
| Bole Tick Virus 2 | 2171 | Unclassified dimarhabdovirus 1 | Ticks | 38.19 | Hyalomma asiaticum | Isfahan virus (38.1%) |
| Huangpi Tick Virus 3 | 2193 | Unclassified dimarhabdovirus 1 | Ticks | 15.81 | Haemaphysalis doenitzi | Eel virus European X (40%) |
| Tacheng Tick Virus 3 | 2182 | Unclassified dimarhabdovirus 1 | Ticks | 96.30 | Dermacentor marginatus | Eel virus European X (39.8%) |
| Taishun Tick Virus | 2226 | Unclassified dimarhabdovirus 1 | Ticks | 24.56 | Haemaphysalis hystricis | Vesicular stomatitis Indiana virus (36.6%) |
| Wuhan Tick Virus 1 | 2191 | Unclassified dimarhabdovirus 1 | Ticks | 119.92 | Rhipicephalus microplus | Eel virus European X (38.3%) |
| Wuhan Insect Virus 7 | 2120 | Unclassified dimarhabdovirus 2 | Insect mix 4 | 241.7 | Hyalopterus pruni OR Aphelinus sp | Isfahan virus (42.6%) |
| Lishi Spider Virus 2 | 2201 | Unclassified mononegavirus 1 | Spiders | 5.57 | Unidentified Araneae | Maize fine streak virus (19.6%) |
| Sanxia Water Strider Virus 4 | 2108 | Unclassified mononegavirus 1 | Water striders | 4767.82 | Unidentified Gerridae | Orchid fleck virus (20.5%) |
| Tacheng Tick Virus 6 | 2068 | Unclassified mononegavirus 1 | Ticks | 17.92 | Argas miniatus | Maize mosaic virus (20.6%) |
| Shuangao Fly Virus 2 | 1966 | Unclassified mononegavirus 2 | Insect mix 1 | 25.94 | Psychoda alternata | Midway virus (21.3%) |
| Xincheng Mosquito Virus | 2026 | Unclassified mononegavirus 2 | Mosquito Hubei | 400.12 | Anopheles sinensis | Midway virus (19.2%) |
| Wenzhou Crab Virus 1 | 1807 | Unclassified mononegavirus 3 | Crabs and barnacles | 382.29 | Capitulum mitella, Charybdis japonica, Charybdis lucifera | Midway virus (22.2%) |
| Tacheng Tick Virus 7 | 2215 | Unclassified rhabdovirus 1 | Ticks | 35.86 | Argas miniatus | Orchid fleck virus (24.5%) |
| Jingshan Fly Virus 2 | 1970 | Unclassified rhabdovirus 2 | True flies | 4.43 | Sarcophaga sp | Maize fine streak virus (23.4%) |
| Sanxia Water Strider Virus 5 | 2264 | Unclassified rhabdovirus 2 | Water striders | 4373.68 | Unidentified Gerridae | Northern cereal mosaic virus (22.6%) |
| Shayang Fly Virus 3 | 2231 | Unclassified rhabdovirus 2 | True flies | 27.73 | Chrysomya megacephala, Atherigona orientalis | Maize fine streak virus (22.6%) |
| Shuangao Bedbug Virus 2 | 2207 | Unclassified rhabdovirus 2 | Insect mix 2 | 16.29 | Cimex hemipterus | Maize fine streak virus (22.5%) |
| Shuangao Insect Virus 6 | 2088 | Unclassified rhabdovirus 2 | Insect mix 1 | 14.37 | Unidentified Diptera, Abraxas tenuisuffusa | Potato yellow dwarf virus (21.2%) |
| Wuhan Ant Virus | 2118 | Unclassified rhabdovirus 2 | Insect mix 3 | 169.79 | Camponotus japonicus | Lettuce necrotic yellows virus (21.4%) |
| Wuhan Fly Virus 3 | 2230 | Unclassified rhabdovirus 2 | True flies | 6.00 | Musca domestica, Sarcophaga sp | Maize fine streak virus (21.9%) |
| Wuhan House Fly Virus 2 | 2233 | Unclassified rhabdovirus 2 | True flies | 221.04 | Musca domestica | Northern cereal mosaic virus (23.4%) |
| Wuhan Mosquito Virus 9 | 2260 | Unclassified rhabdovirus 2 | Mosquito Hubei | 56.19 | Culex tritaeniorhynchus, C. quinquefasciatus, Aedes sp | Persimmon virus A (23.2%) |
| Wuhan Louse Fly Virus 11 | 2110 | Vesiculovirus like | Insect mix 2 | 6.11 | Unidentified Hippoboscidae | Vesicular stomatitis Indiana virus (52.9%) |