Figure 3. Posterior Hox genes can regulate cell ingression in a collinear fashion.
(A) Consecutive electroporation protocol. The ratio of the green domain (green bar, Hox expressing) over the red domain (red bar, control vector) measures the ingression delay. (B) Embryos consecutively electroporated first with Cherry and then with Venus together with control, Hoxa9, Hoxc11, or Hoxb13 vectors. Arrowheads: anterior boundary of Cherry (red) and Venus (green) domains. (C) Ratio of Venus over Cherry domains for posterior Hox genes. Dots: electroporated embryos. Bar indicates the mean. Stars: p-value of two-tailed Student's t-test applied between the different conditions. *p < 0.05; **p < 0.01; ***p < 0.005. Error bars: standard error to the mean (SEM).
Figure 3—figure supplement 1. The posterior Hox genes regulate cell ingression with increasing strength.
(A) Embryos consecutively electroporated with
Hoxd10-Cherry and Hoxc11-Venus
(left) and with Hoxc11-Cherry and
Hoxa13-Venus (right). Arrowheads: anterior boundary
of Cherry (red) and Venus (green) domains. (B) Ratio of
Venus over Cherry domains corresponding to A. This shows
that Hoxa13 retains the cell longer in the epiblast than
Hoxc11 which retains the cell longer than
Hoxd10.