Figure 7.

Caspase inhibition prevents wogonin-induced attenuation of allergic airway inflammation. Following ovalbumin (OVA) sensitization and challenges, mice were treated with vehicle control, wogonin (1 mg at 32 h), z-VAD (5 mg/kg at 32, 36, 40, and 44 h), or combined wogonin and z-VAD, with acquisition and analysis of lungs for histology performed at 48 hours (Day 26). Representative lung tissue sections (hematoxylin and eosin stain) in (A) control/OVA, (B) wogonin-treated, (C) z-VAD-treated, and (D) combined wogonin- and z-VAD-treated animals (all images obtained at ×100 original magnification). (E) Quantification of interstitial and alveolar inflammatory cell infiltration, expressed in arbitrary units (aU) (n = 6 or 7 per group). Data are expressed as mean ± SEM, analyzed by analysis of variance with a Newman-Keuls multiple-comparison test. ***P < 0.001 versus control; ^###P < 0.001 versus wogonin-treated mice.