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. 2015 Feb 27;53(1):77–83. doi: 10.3347/kjp.2015.53.1.77

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© 2015, Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Multiplex PCR pattern on 2% ethidium bromide-stained agarose gel. Lane 1, W. bancrofti; lane 2, D. immitis; lane 3, D. repens. (A) Multiplex PCR confirmed the results of single PCR in El-Nikhila, El-Matiaa villages and Sahel Seleem district. (B) Multiplex PCR detected a positive sample of W. bancrofti (lane 1) that could not be detected by single PCR in El-Nikhila village. (C) Distribution of filarial parasites among different mosquito genera using multiplex PCR. Lane 1, Culex spp.; lane 2, Anopheles spp.; lane 3, Aedes spp. (D) Distribution of filarial parasites among different Culex species in El-Nikhila village. Lane 1, Culex pipiens; lane 2, Culex antennatus; lane 3, Culex pusillus.