Figure 5.

Linearizing the response to d-xylose. The wild type E. coli strain (A), the strain constitutively expressing the high-affinity transporter xylFGH (B), and the strain constitutively expressing the high-affinity transporter xylFGH and lacking the catabolic operon xylAB (C) each harbored the reporter plasmid pUA66-pxylA. The designated strain was back-diluted into M9 minimal medium supplemented with the indicated concentration of d-xylose and grown for 6 h to ABS₆₀₀ ∼ 0.4 prior to flow cytometry analysis. See Figure 3 for more information on the dot plots. Each dot plot is representative of at least three experiments conducted from independent colonies.