Table 1. Response Metrics for Each Alteration of the l-arabinose Utilization Pathway in E. colia.
| alteration | bimodality (Y/n) | δ | EC50 (μM) | η |
|---|---|---|---|---|
| none (WT) | Y | 958 ± 118 | 205 ×/÷ 2.08 | 0.89 ± 0.19 |
| ΔaraBAD | Y | 1023 ± 235 | 9.8 ×/÷ 1.17 | 1.56 ± 0.14 |
| Pcon-araFGH | n | 290 ± 114 | 160 ×/÷ 1.47 | 0.96 ± 0.06 |
| Pcon-araFGH ΔaraBAD | n | 900 ± 288 | 0.54 ×/÷ 1.20 | 1.44 ± 0.07 |
| Pcon-araFGH ΔaraE | n | 262 ± 17 | 56 ×/÷ 1.16 | 1.18 ± 0.03 |
| Pcon-araFGH ΔaraE ΔaraBAD | n | 1440 ± 300 | 0.48 ×/÷ 1.15 | 1.65 ± 0.03 |
| Pcon-araE | Y | 828 ± 282 | 284 ×/÷ 1.02 | 0.75 ± 0.04 |
| Pcon-araE ΔaraBAD | Y | 1351 ± 265 | 0.86 ×/÷ 1.08 | 3.27 ± 0.30 |
| Pcon-araE ΔaraFGH | n | 737 ± 323 | 626 ×/÷ 1.56 | 0.76 ± 0.02 |
| Pcon-araE ΔaraFGH ΔaraBAD | n | 728 ± 407 | 7.5 ×/÷ 1.04 | 1.67 ± 0.09 |
| Pcon-araE ΔaraFGH (ABS600 ∼ 0.004) | n | 517 ± 141 | 10.1 ×/÷ 1.13 | 1.47 ± 0.03 |
| Pcon-araE ΔaraFGH ΔaraBAD (ABS600 ∼ 0.004) | n | 663 ± 128 | 7.1 ×/÷ 1.10 | 1.86 ± 0.01 |
| Pcon-araE ΔaraFGH (ABS600 ∼ 0.04) | n | 371 ± 91 | 21.2 ×/÷ 1.13 | 1.25 ± 0.10 |
| Pcon-araE ΔaraFGH ΔaraBAD (ABS600 ∼ 0.04) | n | 512 ± 78 | 8.6 ×/÷ 1.32 | 1.78 ± 0.08 |
| Pcon-araE ΔaraFGH (ABS600 ∼ 1.0) | n | 1060 ± 161 | 2050 ×/÷ 1.07 | 0.73 ± 0.01 |
| Pcon-araE ΔaraFGH ΔaraBAD (ABS600 ∼ 1.0) | n | 2410 ± 590 | 7.7 ×/÷ 1.19 | 1.64 ± 0.02 |
a
Listed values are the mean and S.E.M. (dynamic range, δ; Hill coefficient, η) or the geometric mean and geometric S.E.M. (effective concentration to achieve 50% induction, EC50) for three independent experiments conducted on separate days. All values reflect cultures grown to ABS600 ∼ 0.4 unless indicated otherwise. The dynamic range was calculated as the ratio of the maximal to minimal mean fluorescence of the entire population, with autofluorescence subtracted from each value. The large error associated with the dynamic range can be attributed to basal levels approaching autofluorescence of cells lacking the reporter plasmid.