Table 1.
The cis -acting elements related to tissue-specific expression identified in pSH4 promoter
| Name | Motif | Position | Putative regulatory function (references in parentheses) |
|---|---|---|---|
| TRAB1 | CAACGTGTGAC | −1775 | Binding site for ABA signal transaction gene TRAB1 (Hobo et al. 1999). |
| OSBZ8 | ACGTGTGCTCCATC | −963 | Binding site for ABA signal transaction gene OSBZ8 (Nakagawa et al. 1996). |
| ACGTATERD1 | ACGT | −1773, −1435, −1037, −961, −846, −819, −679 | ABRE required for dehydration stress and dark-induced senescence (Simpson et al. 2003). |
| DPBFCOREDCDC3 | ACACNNG | −726, −13 | Binding core sequence found in the carrot embryo-specific Dc3 gene promoter, and induced by ABA (Kim et al. 1997). |
| RYREPEATVFLEB4 | CATGCATG | −429 | RY repeat motif, related to ABA-regulated gene expression during late embryo-genesis (Hobo et al. 1999). |
| MYB1AT | WAACCA | −1345, −1201, −75 | MYB recognition site for dehydration-responsive gene and mediated by ABA (Abe et al. 2003). |
| MYCATERD1 | CATGTG | −1252, −1221 | MYC recognition sequence for early responsive to dehydration and mediated by ABA (Simpson et al. 2003). |
| MYCCONSENSUSAT | CANNTG | −1601, −1424, −1415, −1369, −1339, −1252, −1221, −435 | MYC recognition site found in the promoters of the dehydration-responsive gene and mediated by ABA (Abe et al. 2003) |
| MYBGAHV | TAACAAA | −693 | GARC involved in gibberellin signal pathway and sugar suppression (Gubler et al. 1995). |
| WRKY71OS | TGAC | −2062, −1934, −1845, −1768, −1755, −869, −717 | A core of W-box, involved in gibberellin and ABA signaling pathways (Zhang et al. 2004). |
| AMYBOX1 | TAACARA | −693 | Amylase box, conserved sequence found in the promoter of α-amylase gene, regulating specific expression in germinating seeds and callus (Hwang et al. 1998). |
| AMYBOX2 | TATCCAT | −1794 | Amylase box, conserved sequence found in the promoter of α-amylase gene, regulating specific expression in germinating seeds and callus (Hwang et al. 1998). |
| GATABOX | GATA | −1887, −1479, −1236, −813, −503 | Conserved in light-regulated and tissue-specific expression genes (Lam and Chua 1989). |
N = Any base; R = G or A; Y= C or T; W = A or T.