Table I.
Characteristics and activity parameters of platelet concentrates with and without clumping.
| Day of storage* | p value | |||||||
|---|---|---|---|---|---|---|---|---|
|
|
||||||||
| 2 | 5 | 7 | 9 | Main effect | Time | Interaction term | ||
| Platelets (×109/L) | T | 1,003.1±111.5 | 988.1±107.9 | 972.6±106.4 | 946.9±91.8 | 0.698 | < 0.001 | 0.824 |
| C | 1,024.8±100.4 | 1,009.8±96.9 | 982.6±92.4 | 971.6±93.0 | ||||
|
| ||||||||
| MPV (fL) | T | 8.8±0.4 | 8.9±0.3 | 9.0±0.3 | 9.2±0.5 | 0.604 | < 0.001 | 0.381 |
| C | 8.9±0.4 | 9.0±0.4 | 9.1±0.4 | 9.4±0.5 | ||||
|
| ||||||||
| pH (22 °C) | T | 7.12±0.04a | 7.18±0.07 | 7.17±0.07 | 7.07±0.07 | 0.060 | 0.204 | 0.017 |
| C | 7.18±0.07a | 7.20±0.07 | 7.15±0.07 | 7.01±0.09 | ||||
|
| ||||||||
| CD62p (%) | T | 10.9±3.8 | 20.0±6.2 | 17.1±1.4 | 23.9±7.2 | 0.119 | < 0.001 | 0.015 |
| C | 7.4±5.2 | 17.9±4.2 | 19.7±2.3 | 35.1±14.3 | ||||
|
| ||||||||
| Annexin V (%) | T | 2.0±1.1 | 4.0±0.8 | 3.1±1.0 | 3.9±2.0 | 0.137 | < 0.001 | 0.037 |
| C | 1.7±0.8 | 4.1±1.2 | 3.3±1.9 | 7.2±4.0 | ||||
|
| ||||||||
| “Swirling” | T | 2.0±0.0 | 2.0±0.0 | 2.0±0.0 | 1.9±0.3 | 0.369 | 0.038 | 0.571 |
| C | 2.0±0.0 | 2.0±0.0 | 2.0±0.0 | 1.7±0.6 | ||||
|
| ||||||||
| Glucose (mmol/L) | T | 6.2±0.4 | 4.9±0.6 | 3.5±0.6 | 1.9±0.8 | 0.231 | < 0.001 | 0.120 |
| C | 6.3±0.4 | 4.7±0.7 | 3.0±0.7 | 1.2±0.9 | ||||
|
| ||||||||
| Lactate (mmol/L) | T | 6.0±0.6 | 8.8±1.3 | 12.0 ± 1.1 | 15.5 ± 2.0 | 0.555 | < 0.001 | 0.415 |
| C | 5.6±0.4 | 9.1±1.4 | 12.4 ± 1.3 | 16.5 ± 2.5 | ||||
|
| ||||||||
| LDH (U/L) | T | 97.3±17.2 | 103.1±15.8 | 114.4 ± 14.3 | 129.1 ± 24.1 | 0.937 | < 0.001 | 0.150 |
| C | 97.6±23.4 | 115.5±48.8 | 117.2 ± 35.9 | 116.8 ± 27.0 | ||||
*
Data are reported as mean±standard deviation
a
Significant difference on day 2 between groups (p=0.007).
T: Test platelet concentrates (n=12); C: Control platelet concentrates (n=10); MPV: mean platelet volume; LDH: lactate dehydrogenase.