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Purification of YGL157w protein. (A) Purification steps followed by SDS-PAGE. Proteins were separated by SDS-PAGE and visualized by EzStainAqua staining: lane 1, protein molecular size markers; lane 2, crude extract; lane 3, Chelating Sepharose Fast Flow column chromatography pool; lane 4, HiLoad 26/60 Superdex 200 pg column pool. (B) Determination of molecular mass using gel filtration chromatography.
