Table 1.

Primary antibodies and antigen retrieval methods used in immunohistochemical evaluation

Immunohistochemistry
Primary antibody	Clonality	Antigen unmasking	Dilution	Producer
Ki-67, clone MIB-1	Monoclonal	Microwave, citrate buffer, pH 6.0	1:50a	Dako
Active caspase 3	Polyclonal	Water bath, citrate buffer, pH 6.0	1:800b	Cell Signaling
Active caspase 9	Polyclonal	Microwave, EDTA buffer, pH 8.0	1:40a	Novocastra

^a1-h incubation, RT.

^bOvernight incubation, 4°C.