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(a): Multiplex PCR assay for blaOXA and blaSHV. Lane 1, 100-bp DNA ladder; lane 2, positive control for SHV gene (237 bp); lane 3, positive control for OXA gene (813 bp); lanes 4,5,6,7 clinical isolates of ESBL positive (blaOXA); lanes 9,10, clinical isolates of ESBL positive for both blaSHV and blaOXA. (b): Single PCR assay for blaTEM. Lane 1, 100-bp DNA ladder; lanes 2,3, positive control for TEM gene (1080 bp); lanes 4-10, clinical isolates of ESBL negative (blaTEM).
