Figure 4.

Response of EDNRA and EDNRA p.Tyr129Phe to EDN1 and EDN3 In Vitro

After transfection of MC3T3-E1 cells with an expression vector encoding either wild-type EDNRA or EDNRA p.Tyr129Phe, cells were treated with vehicle, EDN1, or EDN3 and then RNA collected for qRT-PCR analysis of Dlx5 and Hand2 expression. All treatments were performed in the presence of the EDNRB antagonist BQ788 to exclude expression changes due to EDNRB signaling. Each condition represents an average of results from three separate transfection experiments. Error bars represent standard error of the mean. Results are normalized to vehicle within each expression assay (Dlx5 or Hand2). ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; n.s., not statistically significant (p > 0.05). Statistical test was a two-tailed t test (assuming equal variance).