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. Author manuscript; available in PMC: 2016 Feb 9.
Published in final edited form as: Dev Cell. 2015 Feb 9;32(3):373–386. doi: 10.1016/j.devcel.2014.12.025

Figure 1. A single histone replacement transgene rescues deletion of the endogenous histone locus.

Figure 1

(A) Schematic of the endogenous replication-dependent histone locus. Breakpoints of the deficiency are indicated by vertical arrows. Each triangle represents a single 5kb histone repeat unit, which was cloned and multimerized into a BAC vector for transgenesis. MCS: Multiple Cloning Site; w+: mini-white cassette; attB: site-specific recombination sequence. (B) Confocal images of cycle 15 embryos stained with antibodies for phospho-histone H3 (red) and DAPI (blue). Genotypes (top to bottom): wild type (yw); homozygous histone deletion (ΔHisC;+); homozygous histone deletion with two copies of the 12× wild type histone transgene (ΔHisC;12×WT). (C) Southern blot of SalI/XhoI digested genomic DNA from 12×WT and wild type (yw) flies, hybridized with an H2A probe. (D) Table of viability tests for various wild type histone arrays. The p-value for the chi-square test is shown. See also Figure S1.