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. 2015 Mar 31;15(3):e24343. doi: 10.5812/hepatmon.24343

Figure 3. hTERT-Specific SECs Efficiently Decreased the Telomerase Activity in HepG2 Cells.

Figure 3.

A) Target sites specific to hTERT B) HepG2 cells were transfected with the indicated SECs. Forty-eight hours later, the telomerase activity was determined by TRAP silver staining analysis. C) Inhibition rate of RNAi, Inhibition rate of RNAi (%) = the relative telomerase activity of the negative control group-the relative telomerase activity of the experimental group; relative telomerase activity = (scanning gray value of the experimental group/scanning gray value of the negative control group) × 100%. 1-8: SEC-1-8; M, pBR322 DNA/Mspl Marker. Mock, calcium phosphate reagent only; NC, negative control SEC; B, Blank control.