Table 1. Primers Used in This Study a.
| Name | Sequence | Targeting Location |
|---|---|---|
| H1F | 5’-GAA TTC GAA CGC TGA CGT C-3’ | H1 forward |
| U6F | 5’-GAG AAG CAT GAA TTC CCC AGT-3’ | U6 forward |
| H1R-1 | 5’-AAG ATG GCC TTC TCA CAG ATT TTT GTG GTC TCA TAC AGA AC-3’ | 1778-1796 |
| U6R-1 | 5’-TCT GTG AGA AGG CCA TCT TCT TTT CGC GTC CTT TCC ACA AGA TA-3’ | 1778-1796 |
| H1R-2 | 5’-GGA GCA AGT TGC AAA GCA TTT TTT GTG GTC TCA TAC AGA AC-3’ | 1800-1818 |
| U6R-2 | 5’-ATG CTT TGC AAC TTG CTC CCT TTT CGC GTC CTT TCC ACA AGA TA-3’ | 1800-1818 |
| H1R-3 | 5’-GGG CTG CGG CCG ATT GTG ATT TTT GTG GTC TCA TAC AGA AC-3’ | 1943-1961 |
| U6R-3 | 5’-TCA CAA TCG GCC GCA GCC CCT TTT CGC GTC CTT TCC ACA AGA TA-3’ | 1943-1961 |
| H1R-4 | 5’-GCG TTT GGT GGA TGA TTT CTT TTT GTG GTC TCA TAC AGA AC-3’ | 2650-2668 |
| U6R-4 | 5’-GAA ATC ATC CAC CAA ACG CCT TTT CGC GTC CTT TCC ACA AGA TA-3’ | 2650-2668 |
| H1R-5 | 5’-GGA AGA CAG TGG TGA ACT TTT TTT GTG GTC TCA TAC AGA AC-3’ | 2760-2778 |
| U6R-5 | 5’-AAG TTC ACC ACT GTC TTC CCT TTT CGC GTC CTT TCC ACA AGA TA-3 | 2760-2778 |
| H1R-6 | 5’-GCC TGT TTC TGG ATT TGC ATT TTT GTG GTC TCA TAC AGA AC-3’ | 3009-3027 |
| U6R-6 | 5’-TGC AAA TCC AGA AAC AGG CCT TTT CGC GTC CTT TCC ACA AGA TA-3’ | 3009-3027 |
| H1R-7 | 5’-GGG AAT TTG GAG TGA CCA ATT TTT GTG GTC TCA TAC AGA AC-3’ | 3878-3896 |
| U6R-7 | 5’-TTG GTC ACT CCA AAT TCC CCT TTT CGC GTC CTT TCC ACA AGA TA-3’ | 3878-3896 |
| H1R-8 | 5’-GAG GTG CTG TGG GAG TAA ATT TTT GTG GTC TCA TAC AGA AC-3’ | 3964-3982 |
| U6R-8 | 5’-TTT ACT CCC ACA GCA CCT CCT TTT CGC GTC CTT TCC ACA AGA TA-3’ | 3964-3982 |
| H1R-NC | 5’-GTG TGA TGT GTC GGT ATT CTT TTT GTG GTC TCA TAC AGA AC-3’ | Negative control |
| U6R-NC | 5’-GAA TAC CGA CAC ATC ACA CCT TTT CGC GTC CTT TCC ACA AGA TA-3’ | Negative control |
a The TTTTT and CTTTT flanking the underlined sequences served as the U6 promoter and H1 promoter termination signals, respectively.