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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Bone. 2014 Sep 6;69:30–38. doi: 10.1016/j.bone.2014.07.038

Fig. 5.

Fig. 5

Fibulin-1 null calvariae express reduced levels of Osterix mRNA. A shows Runx2 levels determined by qPCR analysis of mRNA isolated from E17.5 wild-type (WT) and Fbln1 null (KO) calvariae. B shows levels of the Osterix (Osx) mRNA determined by qPCR analysis of RNA isolated from E16.5 wild-type (WT), Fbln1 heterozygous (HT) and Fbln1 null (KO) calvariae. C shows Osx levels determined by qPCR analysis of mRNA isolated from E17.5 wild-type (WT) and Fbln1 null (KO) calvariae. The graphed values show mean relative amounts of mRNA based on WT (n = 3), HT (n = 6), and KO (n = 3) for E16.5 calvariae and WT (n = 9) and KO (n = 4) for E17.5 calvariae. P value was calculated using one-way ANOVA with Tukey’s post hoc test for B and a Students t-test for A and C.