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. 2015 Mar 28;5:18. doi: 10.1186/s13550-015-0097-z

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© Badar et al.; licensee Springer. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Tricistronic hNET vector design and ASP ⁺ -guided FACS. Three hNET encoding vectors were designed and transduced into SupT1 cells; hNET.l.dCD34 (vector 1), SupT1/FLuc.2A.RQR8.2A.hNET (vector 2), SupT1/hNET.2A.FLuc.2A.RQR8 (vector 3). Transduced (grey lines) and non-transduced (blue lines) cell populations were stained with ASP⁺ followed by flow cytometry (left column). ASP⁺-guided FACS was performed by gating on the brightest 5% within each of the three cell populations (right column).