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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Neurotox Res. 2015 Mar 1;27(4):368–383. doi: 10.1007/s12640-015-9521-4

Figure 2.

Figure 2

Time course for appearances of γH2AX foci in normal fibroblast and SH-SY5Y cells after CPT treatment. Cells were treated with CPT (10 μM) for 15, 30, 60, 90 and 120 min, then IFAs were performed. The top panel shows images of IFA staining of normal fibroblast cells (A, n=3) and SH-SY5Y cells (B, n=3). The bottom panel presents a quantitative analysis of the percentage of γH2AX-positive cells after CPT exposure in normal fibroblast (C) and SH-SY5Y cells (D), representing averages obtained from 3 separate experiments. Images were taken with 60× magnification. At least 200 cells were counted from three random chosen views, from which the positive cell number represented the result from each experiment. *p<0.01, **p<0.0001, compared to the group of 0 min.