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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Neurotox Res. 2015 Mar 1;27(4):368–383. doi: 10.1007/s12640-015-9521-4

Figure 5.

Figure 5

The IFA measurement for γH2AX foci or p-p53ser15-positive fibroblast cells after CPT wash–out. Cells were treated with CPT (10 μM) for 2 h, then CPT was washed away. Cells were allowed to recover in fresh prepared media without CPT for 24 (R24) or 48 (R48) h. After 2 h CPT treatment, significant γH2AX foci (A)were found in nuclei, and the level of p-p53ser15 (B) was increased in nuclei. The numbers of γH2AX- or p-p53ser15-positive cells were reduced within 24 h after CPT wash-out. The images were taken with 60× magnification. Blue: DAPI; red: γH2AX; green: p-p53ser15. Con: control group treated with vehicle. R24 h and R48 h: after CPT was washed away, cells continued to grow in media without CPT for 24 or 48 h. DAPI: 4’,4-diamidino-2-phenylindole, a fluorescent dye that strongly binds to DNA as a nuclear counterstain.