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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Neurotox Res. 2015 Mar 1;27(4):368–383. doi: 10.1007/s12640-015-9521-4

Figure 6.

Figure 6

The IFA measurement for γH2AX foci or p-p53ser15-positive cells in SH-SY5Y cells after CPT wash–out. Cells were treated with CPT (10 μM) for 2 h. After CPT was washed away, cells continued to grow in media without CPT for 24, 48, 72 or 96 h. The numbers of γH2AX-(A) and p-p53ser15-positive (B) cells were reduced within 72 h after CPT wash-out in SH-SY5Y cells. Analytical data of percentage of γH2AX- or p-p53ser15-positive cells are shown in C and D, representing averages obtained from 3 separate experiments. At least 200 cells were counted from three random chosen views, from which the positive cell number represented the result from each experiment. *p<0.0001, compared to the control (con); #p<0.05, ##p<0.0001, compared to the CPT group; &p<0.05, &&p<0.001, &&&p<0.0001, compared to R24 h group. R72 h and R96 h: after CPT was washed away, cells continued to grow in media without CPT for 72 or 96 h. See Fig. 5 for other abbreviation.