Figure 7.

The protein levels of γH2AX and p-p53ser15were decreased within 72 h in SH-SY5Y cells after wash-out of CPT. SH-SY5Y cells were treated with CPT (10 μM) for 2 h, then CPT was washed away, and cells continued to grow in media without CPT for 24 h , 48 h and 72 h. Then western blot analyses were performed. A and B show autoradiographsof γH2AX and p-p53ser15proteins obtained by western blotting. Quantity analysis data are shown in C and D, representing averages obtained from 6 separate experiments.#p<0.001, ##p<0.0001, compared to the control; *p<0. 0001, compared to the CPT group; &p<0.05, &&p<0.001, &&&p<0.0001, compared to the R24 h group. See Figs. 5 and 6 for abbreviations.