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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Neurotox Res. 2015 Mar 1;27(4):368–383. doi: 10.1007/s12640-015-9521-4

Figure 9.

Figure 9

CPT induces DDR in DBH-positive cultured neurons. Cultured LC neurons were treated with CPT (10 μM) for 2 h, then continued to grow in the media without CPT for 24 h, 48 h and 72 h. Only DBH-positive cells were counted. A: Significant γH2AX foci were found in nuclei after CPT treatment. The number of γH2AX-positive cells was reduced gradually within 72 h. Images were taken with 60× magnification. Percentage of γH2AX positive cells were shown in (B), representing averages obtained from 4 separate experiments. At least 100 cells from three random chosen views were counted. C: Enlarged images from yellow boxes in A showγH2AX foci in DBH-positive cells, where yellow arrows indicated γH2AX foci in nuclei. Blue: DAPI; Green: DBH; Red: γH2AX. *p<0.05, **p<0.001, ***p<0.0001, compared to the control; #p<0.0001, compared to the CPT group. See Figs. 5 and 6 for abbreviations.