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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Anaerobe. 2015 Jan 7;32:98–104. doi: 10.1016/j.anaerobe.2015.01.002

Table 3.

Fold change of cprA gene expression analyzed by alkaline phosphatase activity and qRT-PCR in C. difficile grown with and without nisin.

Strain Relevant Genotype Nisin 0.5 µg ml−1/
BHISa,d 		Nisin 5 µg ml−1/
BHISb,d 		Nisin 5 µg ml−1/
Nisin 0.5 µg ml−1c,d
AP qRT-PCR AP qRT-PCR AP qRT-PCR
MC488 Tn916:phoZ 1.1±0.1 89.3±10.5 1.0±0.1 162.7±11.7 1.0±0.0 1.8±0.1
MC489 Tn916::PcprA::phoZ 17.3±1.1 84.7±2.6 32.5±7.9 193.0±27.1 2.7±0.3 2.3±0.4
MC448 pphoZ 1.2±0.1 110.0±8.3 1.5±0.2 201.3±18.0 1.2±0.1 1.8±0.1
MC486 pPcprA::phoZ 23.3±2.9 78.5±5.4 48.3±1.4 152.0±19.0 2.2±0.3 1.9±0.2
a

Fold change of alkaline phosphatase (AP) activity or transcript levels (qRT-PCR) in cells grown in 0.5 µg ml−1 nisin to BHIS medium with no supplement.

b

Fold change of alkaline phosphatase (AP) activity or transcript levels (qRT-PCR) in cells grown in 5 µg ml−1 nisin to BHIS medium with no supplement.

c

Fold change of alkaline phosphatase (AP) activity or transcript levels (qRT-PCR) in cells grown in 5 µg ml−1 nisin to 0.5 µg ml−1 nisin.

d

The means and standard errors of the means of three biological replicates are shown.