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(A) Schematic diagram indicating the structure of the mouse Ift46 gene and the gene-targeting strategy. The Ift46 mutant mice were generated by targeting exon 4. Genotyping primers are shown in the map P1–P3 (arrows). (B) Genotyping of Ift46 mutants at E9.5. The wild-type allele, Ift46 WT, produces a 161 bp fragment and the mutated allele, Ift46 MT, produces a 620 bp PCR fragment.
