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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Microbes Infect. 2015 Jan 8;17(4):275–284. doi: 10.1016/j.micinf.2014.12.008

Figure 2. Deficiency in Atg5 in bone marrow-derived dendritic cells does not diminish IL-12 p70, IL-1β or TNF-α production during incubation with T. gondii-reactive CD4+ T cells.

Figure 2

Dendritic cells transduced with lentiviral vectors encoding Atg5 shRNA or control shRNA were infected with T. gondii (MOI 1:1 tachyzoites per cell) or were incubated T. gondii lysate antigen (TLA; 10 μg/ml). CD4+ T cells were isolated from T. gondii-immunized B6 mice and were co-cultured dendritic cells for 24 h. IL-12 p70, IL-1β and TNF-α production were assessed by ELISA. Results are shown as the mean + SEM and representative of 2 independent experiments.