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. Author manuscript; available in PMC: 2015 Apr 4.
Published in final edited form as: Science. 2014 Nov 20;348(6230):1258699. doi: 10.1126/science.1258699

Fig. 3. Requirements for maintenance of the initiator-independent silent state.

Fig. 3

(A) Establishment and maintenance require HP1 proteins (Swi6 and Chp2) and HDACs (Clr3 and Sir2). (B) Maintenance of ectopic silencing in epelΔ cells does not require Dicer (Deri) as indicated by the growth of red, dcrlΔ cells on +tet medium. (C) Maintenance of ectopic silencing in epelΔ cells does not require Argonaute (Agol) as indicated by the growth of red, agolΔ cells on +tet medium. (D) Either deleting epel (epelΔ) or mutations in its active site (epel-K314A or epel-H297A) allow maintenance of the off state after release of the TetR-Clr4-I initiator. (E) Replacement of clr4+ with clr4ΔCD, encoding Clr4 lacking the chromodomain, abolishes initiator-independent silencing.