Table 2.
Morphological, biochemical and chemotaxonomic properties that are useful in the differentiation of strain M6.X2T and the type species of its close relatives.
| Characteristic | Ezakiella | Anaerococcus | Anaerosphaera | Finegoldia | Gallicola | Helcococcus | Murdochiella | Parvimonas | Peptoniphilus |
|---|---|---|---|---|---|---|---|---|---|
| Spore formation or thermotolerant cell |
− | − | + | − | − | − | − | − | − |
| Fermentation End Product (PYG) |
A, F | A, B L, | A, B | A | A, B | L, A | L | A | A, B |
| Metabolism | Obligate anaerobe |
Obligate anaerobe |
Obligate anaerobe |
Obligate anaerobe |
Obligate anaerobe |
Facultative anaerobe |
Obligate anaerobe | Obligate anaerobe |
Obligate anaerobe |
| Production of Indole |
+ | − | − | − | w | − | + | − | + |
|
Fermentation of: Mannose Raffinose |
− − |
− w |
− − |
− − |
− − |
+ + |
w w |
− − |
− − |
| Activity of: Alkaline phosphatase Arginine arylamidase Histine arylamidase Leucine arylamidase Proline arylamidase Phenylalanine arylamidase Pyroglutamic acid arylamidase |
+ + + + − + + |
− + + w − w + |
nd nd nd nd nd nd nd |
+ + w + − − + |
− − − − − − − |
− − − − − + − |
− + + + + + − |
+ + + + + + + |
− + + − − − − |
| API rapid 32A profile |
0000657707 | 5444017305 | nd | 2000456605 | nd | 0517004020 | 2006272705 | 2000477707 | 2000210001 |
| Cell-wall murein | Lys | Lys, D-Glu | Lys | Lys, D-Asp | D-Asp | D-Asp | ND | Lys | Orn, D-Glu |
| DNA G + C content (mol%) |
38.4 | 30-35 | 32.5 | 32-34 | 27-34 | ND | ND | 27-28 | 30-34 |
| Source | Human feces | Human intestine, clinical material, vaginal discharges, ovarian, peritoneal and other bodily abscesses, skin, and nasal passage |
Methanogenic cattle manure reactor |
Vagina, oral cavity and other bodily abscesses |
Chicken feces | Skin, human clinical material, sheep |
Human clinical material |
Oral cavity and human abscesses |
Human intestine and clinical material, vaginal discharges, ovarian, peritoneal and other bodily abscesses, cattle mastitis and swine manure |
Data: Ezakiella peruensis (M6.X2T), this study; Anaerococcus [31]; Anaerosphaera[14]; Finegoldia[32]; Gallicola[31]; Helcococcus[15]; Murdochiella[16]; Parvimonas[33]; Peptoniphilus[31]. Additional data obtained from de Vos et al., (2009) and the CCUG web site. (www.ccug.se)
+, positive; -, negative; nd, no data; A, acetate; B, Butyrate, F, Formate, L, Lactate; m-Dpm, meso-diaminopimelic acid