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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Membr Biol. 2014 Oct 4;248(3):383–394. doi: 10.1007/s00232-014-9734-0

Fig. 6.

Fig. 6

Determination of the free energy of bilayer insertion chaperoned by FTAC-C6. A combination of FCS methodology and chaperoned membrane insertion protocol utilizing the fluorinated surfactant FTAC-C6 (described in (Posokhov et al., 2008a)). (Top Panel) Lipid titration isotherms for diphtheria toxin T-domain WT in the presence of different concentrations of the fluorinated surfactant FTAC-C6 at pH 4.5. (Bottom panel) Thermodynamic analysis of the bilayer insertion chaperoned by FTAC-C6. Addition of the surfactant results in gradual linear decrease in ΔG. A true value of ΔG of insertion is estimated from the linear extrapolation of the data to zero concentration of the surfactant. The lipid compositions are color coded, and concentration of FTAC-C6 and pH values are coded by symbol shape.