Table 1.

Thermodynamic parameters for the thermal unfolding of the T-domain determined by DSC and CD. The ΔG° values were calculated following the Gibbs–Helmholtz equation and assuming the ΔCp value of 1.44kcal/molK-1 obtained in the DSC experiment.

	Differential scanning calorimetry			Circular dichroism
pH	Tm, °C	ΔH°, kcal/mol	ΔG°, kcal/mol	Tm, °C	ΔH°, kcal/mol	ΔG°, kcal/mol
8*	74 ± 1	94 ± 1	8.0 ± 0.2	75 ± 1	95 ± 7	8.2 ± 0.6
6.5	69 ± 1	86 ± 1	6.8 ± 0.2	74 ± 1	84 ± 6	6.6 ± 0.5
6*	n.d.	n.d.	n.d.	58 ± 1	78 ± 6	5.3 ± 0.4

^*The parameters at pH 8 and pH 6 from the CD measurements were previously published (Vargas-Uribe et al., 2013a).