Skip to main content
. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: J Immunol. 2014 Nov 14;193(12):6152–6160. doi: 10.4049/jimmunol.1303422

Figure 6. Suppression of Th1 and Th17 differentiation by C/EBPα.

Figure 6

(A) Naive CD4+ T cells derived from OT-II TCR Tg mice were transduced with retroviruses carrying pGFP-RV(−) and pGFP-RV-Cebpa (C/EBPα) with primary stimulation by peptide-loaded APCs under Th1 skewing culture. After 4 day, transduced T cells were isolated by their expression of the indicator GFP. IFN-γ expression by re-stimulated Th1 cells was detected by ICS (upper left) and ELISA (upper right). mRNA expression of Cebpa, Ifng and Tbx21 was measured by quantitative PCR (lower). (B) Retroviral transduction of C/EBPα was performed under Th17 skewing condition. IL-17 expression by re-stimulated Th17 cells was detected by ICS (upper left) and ELISA (upper right). mRNA expression of Il17a, Il17f, and Rorc was measured by quantitative PCR (lower). Data are a representative of four independent experiments with similar results.