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Cancer stem cells are enriched in established gemcitabine-resistant cells. A: Panc1-RG cells were established by long-term step-wise gemcitabine treatment. WST assay showed that Panc1-RG cells exhibited gemcitabine resistance compared with Panc1-P cells; B: Flow cytometric analysis showing a higher ratio of CD24+/CD44+ cells in Panc1-RG cells compared with Panc1-P cells. All results are expressed as mean ± SD; aP < 0.05 vs Panc1-P cells.
