Figure 3.

Fucosylation is enhanced in cancer stem cell-like cells fractions of pancreatic cancer cells. A: Phase-contrast images of monolayer (left panel) and sphere cells (right panel) derived from Panc1 cells; B: Results of flow cytometric analysis in monolayer and sphere Panc1 cells using CD24 and CD44 antibodies. CD24⁺/CD44⁺ cells were increased in sphere cells compared with monolayer cells; C: Aleuria aurantia lectin (AAL) or Aspergillus oryzae lectin (AOL) lectin blot of monolayer and sphere Panc1 cells; D: AAL or AOL lectin blot of CD24^high/CD44^high or CD24^low/CD44^low Panc1 cells obtained by cell sorting. Each region is indicated as a square field in (B); E: Comparison of sphere cell-forming ability between Panc1-P and Panc1-RG; F: AAL or AOL lectin blot of monolayer and PSN-1 cells. Results are expressed as mean ± SD; ^bP < 0.01 vs Panc1-P cells. CBB: Coomassie brilliant blue.