Table 1.
Genes | Analysis | 5'-Sequence-3' |
FUT1 | RT-PCR | F: AGACTTTGCCCTGCTCACAC |
R: TGAAGTTGGCCAGGTAGACAG | ||
FUT2 | RT-PCR | F: CAGATGCCTTTCTCCTTTCC |
R: ACTCCCACATGGCTTGAATC | ||
FUT3 | RT-PCR | F: CTGGATCTGGTTCAACTTGG |
R: CGGTAGGACATGGTGAGATTG | ||
FUT4 | RT-PCR | F: GGGTTTGGATGAACTTCGAG |
R: AGCCATAAGGCACAAAGACG | ||
FUT8 | RT-PCR | F: ATCCTGATGCCTCTGCAAAC |
R: GGGTTGGTGAGCATAAATGG | ||
GMDS | RT-PCR | F: TGGAGGCTATGTGGTTGATG |
R: CAAATTCCCGGACACTATGG | ||
FX | RT-PCR | F: ACACGTCATCCATCTTGCTG |
R: AGGACGTTGTCGTTCATGTG | ||
SLC35C1 | RT-PCR | F: GGTGTGGCCTTCTACAATGTG |
R: ATGATGATACCGCAGGTGAG | ||
HNF4α | RT-PCR | F: CATCTTCTTTGACCCAGATGC |
R: CGTTGATGTAGTCCTCCAAGC | ||
IL-6 | RT-PCR | F: ATGCAATAACCACCCCTGAC |
R: GCGCAGAATGAGATGAGTTG | ||
RPL4 | RT-PCR | F: GACTTAACACACGAGGAGATGC |
R: GCATGCTGTGCACATTTAGG | ||
FUT1 | shRNA | GGTAATCAGATGGGACAGTAT |
Negative control | shRNA | GGAATCTCATTCGATGCATAC |
SLC35C1 | shRNA | CUACUUAUAGACCCAAUCATT |
Negative control | shRNA | UCUUAAUCGCGUAUAAGGCTT |