FIG. 3.
Immunoblot of FlgR in H. pylori cell extracts. The immunoblot was probed with an antibody directed against the histidine-tagged FlgR protein. Arrow, band corresponding to FlgR. Different amounts of cell extract of wild-type H. pylori were loaded onto lanes 1 to 7 (lane 1, 1 × 109 cells; lane 2, 5 × 108 cells; lane 3, 2 × 108 cells; lane 4, 1 × 108 cells; lane 5, 5 × 107 cells; lane 6, 2 × 107 cells; lane 7, 1 × 107 cells). In lane 8, 109 cells of the mutant H. pylori flgR:cat strain were loaded. Different amounts of purified MBP-FlgR were loaded onto lanes 9 to 14 (lane 9, 200 ng; lane 10, 100 ng; lane 11, 50 ng; lane 12, 25 ng; lane 13, 12.5 ng; lane 14, 6 ng).