FIG. 2.

Replication of Mu and chimeric Mu prophages. Cultures of lysogens were grown and induced as described for Fig. 1. At indicated times after induction, the cultures were assayed for the relative amounts of prophage DNA normalized to a control mal chromosomal locus by a semiquantitative PCR approach, as described in Materials and Methods. Two representative experiments are shown, encompassing data from the positive- and negative-control prophage sequences as well as the five recombinants.