Skip to main content
. 2004 Jul;186(14):4665–4684. doi: 10.1128/JB.186.14.4665-4684.2004

FIG. 1.

FIG. 1.

Confirmation of S. aureus UAMS-1 sarA and ica mutants. (A) Genomic DNA isolated from UAMS-1 (U1) and its corresponding ica or sarA mutants was digested with HpaI and blotted with probes corresponding to an internal fragment of the ica operon or the sarA locus. (B) Dot blot analysis of PNAG expression in S. aureus strain UAMS-1 and its corresponding ica and sarA mutants. Duplicate samples prepared as described in Materials and Methods were spotted onto membranes and analyzed using antiserum raised against S. aureus PNAG.