FIG. 4.

Biofilm formation in coinfection experiments with S. aureus UAMS-1 and its sarA and ica mutants in vivo. Catheters were coinfected with equal numbers of UAMS-1 and its ica mutant (A) or UAMS-1 and its sarA mutant (B). The total number of bacteria in the inoculum and the total number recovered from each catheter was determined by plate count on nonselective medium. The number of bacteria resistant to tetracycline (A) or kanamycin (B) was subsequently determined by transferring individual colonies to selective media. Resistance to tetracycline and kanamycin is indicative of the ica and sarA mutations, respectively. Abbreviations: TetS and TetR, tetracycline sensitive and resistant, respectively; KanS and KanR, kanamycin sensitive and resistant, respectively.