TABLE 3.
N315 ORFa | Common namea | Producta,b | ERc |
---|---|---|---|
Cell envelope and cellular processes | |||
N315-SA1960 | mtlF | PTS system, mannitol-specific IIBC component | 5.46 |
N315-SA1882 | kdpD | Sensor protein KdpD | 5.13 |
N315-SA2311 | Similar to NAD(P)H-flavin oxidoreductase | 2.71 | |
N315-SA1156 | ABC transporter (ATP-binding protein) homolog | 2.68 | |
N315-SA0724 | Similar to cell division inhibitor | 0.496 | |
N315-SA2253 | opp-1C | Oligopeptide transporter putative membrane permease domain | 0.490 |
N315-SA0567 | Similar to iron(III) ABC transporter permease protein | 0.474 | |
N315-SA2216 | Similar to ABC transporter, ATP-binding protein | 0.471 | |
N315-SA0980 | Similar to ferrichrome ABC transporter | 0.471 | |
N315-SA0981 | Similar to ferrichrome ABC transporter | 0.468 | |
N315-SA0592 | tagA | Teichoic acid biosynthesis protein | 0.462 |
N315-SA1935 | hmrA | Similar to amidase | 0.442 |
N315-SA1169 | γ-Aminobutyrate permease | 0.425 | |
N315-SA0243 | tagB | Similar to teichoic acid biosynthesis protein B | 0.423 |
N315-SA0110 | sirB | Lipoprotein | 0.399 |
N315-SA2100 | Similar to autolysin E | 0.394 | |
N315-SA1458 | lytH | N-Acetylmuramoyl-l-alanine amidase | 0.393 |
N315-SA0109 | sirC | Lipoprotein | 0.378 |
N315-SA0106 | lctP | l-Lactate permease homolog | 0.376 |
N315-SA0682 | Similar to ditripeptide ABC transporter | 0.370 | |
N315-SA2053 | Glucose uptake protein homolog | 0.331 | |
N315-SA0479 | nupC | Pyrimidine nucleoside transport protein | 0.315 |
N315-SA0111 | sirA | Lipoprotein | 0.312 |
N315-SA2339 | Similar to antibiotic transport-associated protein | 0.291 | |
N315-SA0566 | Similar to iron-binding protein | 0.287 | |
N315-SA2233 | Similar to integral membrane efflux protein | 0.281 | |
N315-SA0325 | glpT | Glycerol-3-phosphate transporter | 0.281 |
N315-SA2112 | Similar to sodium-dependent transporter | 0.278 | |
N315-SA1025 | mraY | Phospho-N-muramic acid-pentapeptide translocase | 0.265 |
N315-SA0600 | Similar to pyrimidine nucleoside transporter | 0.255 | |
N315-SA1978 | Similar to ferrichrome ABC transporter (permease) | 0.203 | |
N315-SA0010 | azlC | Similar to amino acid permease | 0.161 |
N315-SA2300 | Similar to glucarate transporter | 0.141 | |
N315-SA0691 | sstD | Lipoprotein, similar to ferrichrome ABC transporter | 0.126 |
N315-SA0374 | pbuX | Xanthine permease | 0.089 |
N315-SA0579 | Similar to Na+/H+ antiporter | 0.080 | |
N315-SA0411 | ndhF | NADH dehydrogenase subunit 5 | 0.065 |
N315-SA2302 | stpC | Similar to ABC transporter | 0.046 |
N315-SA2303 | smpC | Similar to membrane-spanning protein | 0.041 |
Information pathways | |||
N315-SA1883 | kdpE | KDP operon transcriptional regulatory protein | 5.42 |
N315-SA2429 | ArgR | Similar to arginine repressor | 3.92 |
N315-SA2296 | Similar to transcriptional regulator, MerR family | 3.72 | |
N315-SA2418 | Similar to two-component response regulator | 2.13 | |
N315-SA0460 | pth | Peptidyl-tRNA hydrolase | 0.490 |
N315-SA0652 | Similar to transcription regulation protein | 0.452 | |
N315-SA1853 | Similar to DNA mismatch repair protein MutS | 0.445 | |
N315-SA1287 | asnS | Asparaginyl-tRNA synthetase | 0.441 |
N315-SA0348 | Similar to transcription terminator | 0.440 | |
N315-SA2358 | Similar to transcriptional regulator (TetR/AcrR family) | 0.382 | |
N315-SA1697 | Similar to protein-tyrosine phosphatase | 0.364 | |
N315-SA1120 | Similar to transcription regulator GntR family | 0.354 | |
N315-SA0298 | pfoR | Similar to regulatory protein PfoR | 0.333 |
N315-SA1550 | tyrS | Tyrosyl-tRNA synthetase | 0.325 |
N315-SA2482 | pcp | Pyrrolidone-carboxylate peptidase | 0.297 |
N315-SA1583 | rot | Repressor of toxins (Rot) | 0.295 |
N315-SA0653 | fruR | Similar to transcription repressor of fructose operon | 0.229 |
N315-SA0904 | Probable ATL autolysin transcription regulator | 0.191 | |
N315-SA1725 | sspB | Staphopain, cysteine proteinase | 0.074 |
Intermediary metabolism | |||
N315-SA0328 | Similar to NADH-dependent FMN reductase | 7.25 | |
N315-SA0122 | butA | Acetoin (diacetyl)reductase | 5.04 |
N315-SA2297 | Similar to GTP-pyrophosphokinase | 3.25 | |
N315-SA1142 | glpD | Aerobic glycerol-3-phosphate dehydrogenase | 2.97 |
N315-SA0016 | purA | Adenylosuccinate synthase | 2.37 |
N315-SA2397 | Similar to pyridoxal-phosphate-dependent aminotransferase | 2.04 | |
N315-SA2001 | Similar to oxidoreductase, aldo/keto reductase family | 2.01 | |
N315-SA1201 | trpD | Anthranilate phosphoribosyltransferase | 0.495 |
N315-SA1685 | mutY | Similar to A/G-specific adenine glycosylase | 0.481 |
N315-SA2111 | Similar to phosphoglycolate phosphatase | 0.475 | |
N315-SA1052 | gmk | Guanylate kinase homolog | 0.462 |
N315-SA2279 | Similar to phosphomannomutase | 0.458 | |
N315-SA0902 | HisC homolog | 0.448 | |
N315-SA0177 | argJ | Arginine biosynthesis bifunctional protein homolog | 0.436 |
N315-SA1310 | ansA | Probable l-asparaginase, gene-ansA | 0.429 |
N315-SA1309 | cmk | Cytidylate kinase | 0.427 |
N315-SA1749 | Similar to aspartate transaminase protein | 0.423 | |
N315-SA2140 | Similar to esterase | 0.412 | |
N315-SA0507 | Similar to N-acyl-l-amino acid amidohydrolase | 0.407 | |
N315-SA0568 | Similar to l-2-haloalkanoic acid dehalogenase | 0.374 | |
N315-SA2213 | bioB | Biotin synthase | 0.373 |
N315-SA0514 | Similar to deoxypurine kinase | 0.356 | |
N315-SA1121 | Similar to processing proteinase homolog | 0.340 | |
N315-SA1203 | trpF | Phosphoriborylanthranilate isomerase | 0.323 |
N315-SA2342 | thgA | Similar to O-acetyltransferase | 0.280 |
N315-SA0511 | Similar to UDP-glucose 4-epimerase-related protein | 0.256 | |
N315-SA1202 | trpC | Indole-3-glycerol phosphate synthase | 0.246 |
N315-SA2395 | ldh | l-Lactate dehydrogenase | 0.213 |
N315-SA0180 | bmQ | Similar to branched-chain amino acid transport system carrier protein | 0.192 |
N315-SA1200 | trpG | Anthranilate synthase component II | 0.120 |
N315-SA1199 | trpE | Similar to anthranilate synthase component I | 0.101 |
N315-SA0373 | xprT | Xanthine phosphoribosyltransferase | 0.077 |
Other functions | |||
N315-SA0899 | sspC | Cysteine protease | 7.24 |
N315-SA0900 | sspB | Cysteine protease precursor | 7.03 |
N315-SA0150 | cap5G | Capsular polysaccharide synthesis enzyme | 4.80 |
N315-SA2006 | Similar to MHC class II analog | 4.69 | |
N315-SA0149 | cap5F | Capsular polysaccharide synthesis enzyme | 4.58 |
N315-SA0148 | cap5E | Capsular polysaccharide synthesis enzyme | 4.05 |
N315-SA0146 | cap5C | Capsular polysaccharide synthesis enzyme | 3.31 |
N315-SA0147 | cap5D | Capsular polysaccharide synthesis enzyme | 3.30 |
N315-SA0145 | cap5B | Capsular polysaccharide synthesis enzyme Cap5B | 2.43 |
N315-SA0144 | cap5A | Capsular polysaccharide synthesis enzyme | 2.34 |
N315-SA0841 | Similar to cell surface protein Map-w | 2.22 | |
N315-SA1709 | Similar to ferritin | 2.18 | |
N315-SA0754 | Similar to lactococcal prophage ps3 protein 05 | 0.498 | |
N315-SA1835 | int | Similar to integrase (pathogenicity island SaPln1), gene = int | 0.431 |
N315-SA1559 | Similar to smooth muscle caldesmon | 0.426 | |
N315-SA0797 | nifU-3 | Similar to nitrogen fixation protein NifU | 0.407 |
N315-SA0780 | Similar to hemolysin | 0.331 | |
N315-SA0746 | nuc | Staphylococcal nuclease | 0.170 |
N315-SA1766 | HP (bacteriophage φN315) | 0.069 | |
N315-SA1775 | Similar to scaffolding protein (bacteriophage φN315) | 0.048 | |
N315-SA1765 | HP (bacteriophage φN315) | 0.042 | |
N315-SA1777 | HP (bacteriophage φN315) | 0.038 | |
N315-SA1771 | HP (bacteriophage φN315) | 0.036 | |
N315-SA1762 | HP (bacteriophage φN315) | 0.036 | |
Similar to unknown proteins | |||
N315-SA0326 | CHP (lactoylglutathione lyase and related lyases) | 7.94 | |
N315-SA0327 | CHP (flavin-dependent oxidoreductases) | 7.50 | |
N315-SA2479 | CHP | 5.04 | |
N315-SA0007 | Predicted sugar kinase | 4.57 | |
N315-SA0380 | CHP (pathogenicity island SaPln2) | 3.07 | |
N315-SA0381 | CHP (pathogenicity island SaPln2) | 2.80 | |
N315-SA1235 | CHP | 2.04 | |
N315-SA1890 | CHP | 2.03 | |
N315-SA0230 | CHP | 2.02 | |
N315-SA0941 | CHP | 0.497 | |
N315-SA0467 | CHP (predicted ATPase of the PP-loop superfamily) | 0.489 | |
N315-SA1838 | CHP (predicted metal-dependent membrane protease) | 0.481 | |
N315-SA2487 | rarD | Similar to RarD protein | 0.462 |
N315-SA1696 | CHP | 0.453 | |
N315-SA1448 | CHP (TPR repeat-containing proteins) | 0.452 | |
N315-SA0329 | CHP | 0.445 | |
N315-SA2328 | CHP (putative effector of murein hydrolase, LrgB) | 0.434 | |
N315-SA0979 | CHP | 0.433 | |
N315-SA1928 | HP | 0.421 | |
N315-SA1601 | crcB | CHP (integral membrane protein) | 0.404 |
N315-SA2377 | CHP | 0.398 | |
N315-SAS081 | CHP (ATPase involved in DNA repair) | 0.391 | |
N315-SA0334 | CHP (Sec-independent protein secretion pathway) | 0.378 | |
N315-SA2305 | CHP | 0.372 | |
N315-SA0840 | CHP (phospholipid-binding protein) | 0.370 | |
N315-SA1903 | CHP | 0.366 | |
N315-SA0543 | CHP (uncharacterized BCR) | 0.353 | |
N315-SA0413 | CHP | 0.332 | |
N315-SA0341 | Similar to low-temperature requirement A protein | 0.329 | |
N315-SA0345 | CHP (methionine synthase I) | 0.324 | |
N315-SA0257 | CHP (SAM-dependent methyltransferases) | 0.323 | |
N315-SA2096 | CHP | 0.320 | |
N315-SA0773 | CHP (predicted membrane protein) | 0.312 | |
N315-SA2212 | Similar to 8-amino-7-oxononanoate synthase | 0.303 | |
N315-SA1252 | CHP (histone acetyltransferase) | 0.298 | |
N315-SA2452 | CHP (domain typically associated with flavoprotein oxygenases) | 0.282 | |
N315-SA0870 | CHP (predicted permease) | 0.267 | |
N315-SA0335 | CHP (Sec-independent protein secretion pathway) | 0.265 | |
N315-SA0556 | CHP | 0.203 | |
N315-SA0753 | CHP (lysine efflux permease) | 0.179 | |
N315-SA2219 | CHP (uncharacterized membrane protein) | 0.133 | |
N315-SA0739 | CHP | 0.129 | |
N315-SAS001 | CHP | 0.128 | |
N315-SA0412 | CHP | 0.078 | |
No similarity | |||
N315-SAS016 | HP | 12.74 | |
N315-SA0883 | HP | 5.97 | |
N315-SA1233 | HP | 2.25 | |
N315-SA0414 | HP | 0.496 | |
N315-SA2126 | HP | 0.495 | |
N315-tRNA12 | tRNA-Pro | 0.491 | |
N315-SA1943 | HP | 0.491 | |
N315-SA1215 | HP | 0.486 | |
N315-tRNA11 | tRNA-Arg | 0.479 | |
N315-SA0613 | HP | 0.470 | |
N315-SA2485 | HP | 0.457 | |
N315-SA0088 | HP | 0.443 | |
N315-tRNA57 | tRNA-Lys | 0.416 | |
N315-tRNA47 | tRNA-Leu | 0.409 | |
N315-SA1607 | HP | 0.402 | |
N315-SA0955 | HP | 0.372 | |
N315-tRNA07 | tRNA-Thr | 0.355 | |
N315-SA0105 | HP | 0.344 | |
N315-SA2118 | HP | 0.335 | |
N315-SA0336 | HP | 0.326 | |
N315-SA0363 | HP | 0.321 | |
N315-SA2055 | HP | 0.321 | |
N315-SA2224 | HP | 0.309 | |
N315-SA2249 | HP | 0.259 | |
N315-SA0749 | HP | 0.233 | |
N315-tRNA06 | tRNA-Val | 0.219 | |
N315-SA0748 | HP | 0.214 | |
N315-SA0889 | HP | 0.179 | |
N315-SA2488 | HP | 0.157 | |
N315-SA1778 | HP (bacteriophage φN315) | 0.089 | |
N315-SA1768 | HP (bacteriophage φN315) | 0.050 | |
N315-SA1770 | HP (bacteriophage φN315) | 0.050 | |
N315-SA1774 | HP (bacteriophage φN315) | 0.046 | |
N315-SA1776 | HP (bacteriophage φN315) | 0.044 | |
N315-SAS060 | HP (bacteriophage φN315) | 0.041 | |
N315-SA1769 | HP (bacteriophage φN315) | 0.040 | |
N315-SA1773 | HP (bacteriophage φN315) | 0.032 | |
N315-SA1772 | HP (bacteriophage φN315) | 0.031 | |
N315-SA1767 | HP (bacteriophage φN315) | 0.027 | |
No N315 ORF | |||
set5 | 100% protein ID Set5, exotoxin 5, and HsdM-like protein gene | 40.43 | |
sspA | 99.4% protein ID S. aureus glutamic acid-specific protease | 7.19 | |
cap8H | 100% protein ID to capsular polysaccharide synthase enzyme Cap8H | 5.11 | |
cap8J | 100% protein ID to capsular polysaccharide synthesis enzyme Cap8J | 4.52 | |
58.1% protein ID malofactic enzyme, Oenococcus oeni bacteria | 3.05 | ||
68.1% protein ID SA0329 CHP | 2.39 | ||
92.8% protein ID MW1748 HP | 2.25 | ||
84.4% protein ID MW0360 HP | 2.16 | ||
96.7% protein ID to MW2134 HP | 0.500 | ||
95.6% protein ID SA2230 | 0.473 | ||
58.4% protein ID BH3950 transposase (10), Bacillus halodurans | 0.467 | ||
49.1% protein ID MW2618 | 0.463 | ||
COL-SA1788 | HP | 0.434 | |
87.6% protein ID MW0584 | 0.423 | ||
COL-SA2299 | HP | 0.421 | |
Similar to splE | 62.6% protein ID serine protease SplE | 0.420 | |
97.7% protein ID to SA1559 | 0.419 | ||
26.9% protein ID MW2498 | 0.418 | ||
COL-SA0866 | HP | 0.411 | |
Serine protease | 55.4% protein ID serine protease SplB | 0.409 | |
96% protein ID MW2325 | 0.387 | ||
95.5% protein ID MW0355 HP | 0.371 | ||
22.2% protein ID SA0283 HP | 0.345 | ||
89.4% protein ID SA0553 CHP | 0.338 | ||
78.2% protein ID MW1720 HP | 0.315 | ||
COL-SA1556 | HP | 0.311 | |
99.4% protein ID to MW0053 CHP | 0.308 | ||
45.9% protein ID BH3950 transposase (10), Bacillus halodurans | 0.290 | ||
97.7% protein ID MW0355 HP | 0.289 | ||
splB | 97.7% protein ID to serine protease SplB | 0.280 | |
79.2% protein ID MW1043 HP | 0.270 | ||
COL-SA2728 | HP | 0.237 | |
99.5% id to SAV1992 HP | 0.209 | ||
37.3% protein ID MW1769 HP | 0.202 | ||
COL-SA1140 | sai-1 | 29-kDa cell surface protein | 0.164 |
85.2% protein ID MW1042 HP | 0.141 | ||
99.5% protein ID MWP018 | 0.138 | ||
99.8% protein ID MWP016 S. aureus plasmid pMW2 | 0.096 | ||
93.4% protein ID SAV1953 φPVL ORF 20 and 21 homolog | 0.079 | ||
Mu50-SAV1953 | φPVL ORF 20 and 21 homolog | 0.071 | |
100% protein ID to MWP017 HP | 0.070 | ||
100% protein ID MW1894 HP | 0.060 | ||
78% protein ID MW1892 HP | 0.055 | ||
83.2% protein ID SA1763 HP | 0.032 | ||
100% protein ID SAP019 HP, S. aureus N315 plasmid N315B | 0.031 |
Based on the published sequence of S. aureus strain N315. For genes not present in N315, the gene name and description given are from the S. aureus strain COL genome, available from The Institute for Genomic Research website (http://www.tigr.org) or by the putative function.
Abbreviations: PTS, phosphotransferase; HP, hypothetical protein; CHP, conserved hypothetical protein; SAM, S-adenosylmethionine; ID, identity.
Normalized values based on the expression ratio (ER), which is defined as the expression level in exponential-phase cells/expression level in stationary-phase cells.