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. 2004 Jul;186(14):4466–4475. doi: 10.1128/JB.186.14.4466-4475.2004

FIG. 2.

FIG. 2.

Biofilm formation by P. aeruginosa strains. (A) The biofilm formation of strains PAO1 (circles), WFPA50 (squares), and WFPA60/pMO011305 (triangles) was assayed at 2-h intervals in a static microtiter plate system (40). Surface-attached cells were stained with crystal violet, the stain was solubilized in ethanol, and the absorbance was analyzed at 540 nm. (B) Biofilm formation of PAO1 (black) and MS2 (white) at 10 h in a static microtiter plate system (40). Bacteria were cultured with the following carbon sources (0.4%): A, LB medium; B, LB medium with glucose; C, 10× glucose; D, succinate; E, glycerol; F, glutamate (F).