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Quantification of bacteria recovered during simultaneous and sequential coinoculation biofilm tubing assaysa
| Strain | Inoculation | Time of harvesting (h) | Fraction of bacteria recovered |
|---|---|---|---|
| PAO1 Smr | Simultaneous | 48 | 0.999 |
| WFPA60 | Simultaneous | 48 | 0.001 |
| PAO1 Smr | Sequential | 48 | 0.999999 |
| WFPA60 | Sequential | 24 | 0.000001 |
At 48 h, the biofilm cells were harvested, resuspended, plated onto selective media, enumerated, and expressed as a percentage of the total population of cells recovered.
