FIG. 2.

RT-PCR of total RNA prepared from P. stutzeri WM567 grown on hypophosphite as the sole P source to determine the operon structure of htx. Lanes a show complete RT reactions; lanes b contain a negative control, with which no reverse transcriptase was added to the reaction mixture; and lanes c contain a PCR-positive control, for which chromosomal DNA was used as the template. Lanes L, 100-bp ladder. The junction sequences amplified are indicated above each set of reactions. For a list of the primers used and the predicted PCR product sizes, refer to Table 2.