TABLE 1.
Oligonucleotide primers used for deletion constructions
| Deletion | Sequence amplified | Primer seta | Restriction enzyme site |
|---|---|---|---|
| Δhtx | Upstream htxA | 5′-GGCGGCGGCACTAGTCTGCCCCGATATCAAGCAAC-3′ | SpeI |
| 5′-GGCGGCGGCGCGGCCGCGTGATGCTCCAAGGTCTTCC-3′ | NotI | ||
| Downstream htxO | 5′-GGCGGCGGCGCGGCCGCATGGCATCGAGTGCTCAAC-3′ | NotI | |
| 5′-GGCGGCGGCGAGCTCGCCGGACATTTGTATACGC-3′ | SacI | ||
| Δptx | Upstream ptxA | 5′-GGCGGCGGCACTAGTGGCTAGCATCACACAGAAACC-3′ | SpeI |
| 5′-GGCGGCGGCGCGGCCGCCCTTGTTACCGCACTGCTTC-3′ | NotI | ||
| Downstream ptxE | 5′-GGCGGCGCGGCCGCGGTGATGGATGGTTGCGAT-3′ | NotI | |
| 5′-GGCGGCGAGCTCCAGCGTGGCGTAGAGCTGCG-3′ | SacI | ||
| Δphn | Upstream phnC | 5′-GGCGCGCCGAGCTCACCAGGCCTGGCGTGTCGGC-3′ | SacI |
| 5′-GGCGCGCCGCGGCCGCTCTGCGCGGCCGGGGAGGT-3′ | NotI | ||
| Downstream phnP | 5′-GGCGCGCCGCGGCCGCCGCTTAGGAGAGAAGCCGAG-3′ | NotI | |
| 5′-GGCGCGCCAACTAGTGTTCGGGCAAGCGCTCCAGC-3′ | SpeI |
a
The sequences of the restriction sites incorporated into the PCR primers are underlined.