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. 2004 Jul;186(14):4730–4739. doi: 10.1128/JB.186.14.4730-4739.2004

TABLE 2.

Oligonucleotide primers used for the amplification of htx junction sequences

Junction amplified Primer set Predicted product size (bp)
htxAB 5′-AAGTGTACGACCACCGGAAC-3′
586
5′-GTCTGGCTAGGCGTTGGAC-3′
htxBC 5′-TCCTTCGAAGTCCAAGATCG-3′
587
5′-CAGCCCCTTTTAGAATGACG-3′
htxCD 5′-CAGCAGGGCAAAGATTATCC-3′
560
5′-CCGGAGACAGTTCATGTATGG-3′
htxDE 5′-GTGCATGCGCTTTACCAG-3′
583
5′-ACAGCGCAATCTCCAGGTCT-3′
htxEF 5′-AGAACGCCGACAAGAAGC-3′
589
5′-TTCGATCGACTGCCCTTG-3′
htxFG 5′-GCATGCCAGGTTGTGAAG-3′
573
5′-GCACCTGCTCGTGTTGAATA-3′
htxGH 5′-GGCAACCGTTTGCTGGAG-3′
581
5′-CACCGGCACATCCATCAG-3′
htxHI 5′-CCGGTGAGGTACTGATGACC-3′
553
5′-AATCAGCGACAGCGTAACCT-3′
htxIJ 5′-CATCAACGGCCACTATGTGA-3′
700
5′-CTGGTACACCATGCCAAAGC-3′
htxJK 5′-CCGCTGCTCTACCTCGAC-3′
591
5′-ACAGTGCAGAAATTGGGTGA-3′
htxKL 5′-GAACTGCTGGCCCTGCTC-3′
556
5′-AACTCCAGCGGGAAGTGC-3′
htxLM 5′-CTGCCTGTGCGGTGACTACT-3′
600
5′-GGGCCTCGTCCAGGTATT-3′
htxMN 5′-GAATCGGCATCGAGATCAAC-3′
584
5′-CAGCGCAGATGAAAGAGTCC-3′
htxN-orf282 5′-GCTTCGCCTACCTCACAGAC-3′
587
5′-AACTGCGCACCAGAGGATAG-3′
orf282-orf344 5′-CTCTTGGCGGAACCTGTATC-3′
569
5′-GAGCGAATGCCTTTGAGAAC-3′